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Today is May 19, 2012, 11:00 am

Once the tissue sample arrives at HSCC, stem cells are released by the action of protease. The process takes approximately 2 hours. The clinician can either use the cells immediately (2) or they could be clonally expanded (1) for future use. The laboratory has been providing storage service for stem cells since January 2009 in the vapor phase of liquid nitrogen. The stem cells could be thawed and use at any time allowing great flexibility in the planning of treatment.

The photograph shows a 50 ml container after density gradient centrifugation. This process is part of the final stage of stem cell isolation.

Mesenchymal stem cells attach to the plastic surface and exhibit spindle shape morphology.

The majority of stem cell transplants have been performed for facial rejuvenation, using mesenchymal stem cells derived from lipoaspirate after liposuction.

A 31 year old lady showed early signs of aging in the forehead. There were volume reduction, drying and coarsening of the skin.

It is 6 months after grafting with lipoaspirate and fat-derived mesenchymal stem cells. The skin texture becomes smooth with reduction of pore size. It becomes more reflective due to an improvement in skin hydration. Mesenchymal stem cells participate in the formation of new blood vessels (3). They also promote angiogenesis and leads to an improvement of skin circulation and hydration (4).

Stem cell transplant performed by HSCC for other indications include:

Breast augmentation (5).
Improvement of eye sight after retinal haemorrhage.
Pain subsides with the return of function in Golfer's elbow and De Quervain's disease.
Improvement of Type 2 Diabetes: HbA1C was greatly reduced.
Immediate termination of acute asthmatic attack with complete resolution of asthma (6).

Stem cell therapy is still in its early stage and the growth potential of this therapy is vast. HSCC has established custom protocols and caters for the specific needs of individual professionals. We welcome interested parties to join us as a member of HSCC.

Reference:

K. Le Blanc, F. Frassoni, L. Ball, F. Locatelli, H. Roelofs, I. Lewis, E. Lanino, B. Sundberg, M. E. Bernardo, M. Remberger, G. Dini, R M. Egeler, A. Bacigalupo, W. Fibbe, O. Ringdén Mesenchymal stem cells for treatment of steroid-resistant, severe, acute graft-versus-host disease: a phase II study Lancet 2008; 371: 1579–86
N. H Riordan , T. E Ichim, Wei-Ping Min , H.Wang , F. Solano , F. Lara , M. Alfaro , J. P. Rodriguez , R. J Harman , A. N Patel , M. P. Murphy , R. R Lee., B. Minev. Non-expanded adipose stromal vascular fraction cell therapy for multiple sclerosis. Journal of Translational Medicine 2009, 7:29doi:10.1186/1479-5876-7-29
Lu, Feng M.D., Ph.D.; Mizuno, Hiroshi M.D.; Uysal, Cagri A. M.D.; Cai, Xiaobo M.D.; Ogawa, Rei M.D., Ph.D.; Hyakusoku, Hiko M.D. Improved Viability of Random Pattern Skin Flaps through the Use of Adipose-Derived Stem Cells Plastic and Reconstructive Surgery: January 2008 - Volume 121 - Issue 1 - pp 50-58
Rigotti G, Marchi A, Galiè M, Baroni G, Benati D, Krampera M, Pasini A, Sbarbati A. Clinical treatment of radiotherapy tissue damage by lipoaspirate transplant: a healing process mediated by adipose-derived adult stem cells. Plast Reconstr Surg. 2007 Apr 15;119(5):1409-22; discussion 1423-4.
K. Yoshimura, K. Sato, N. Aoi, M. Kurita, T. Hirohi, K. Harii Cell-Assisted Lipotransfer for Cosmetic Breast Augmentation:Supportive Use of Adipose-Derived Stem/Stromal Cells Aesth Plast Surg (2008) 32:48–55
C Palmieri, R Gillmore, A Menzies-Gow, S Fishpool, D Robinson, R Shaw, R C Coombes, Resolution of late-onset asthma following high-dose chemotherapy Bone Marrow Transplantation (2003) 32, 847–848.